研究员

重庆市医师协会医学科研实验室建设与管理专业委员会第一届实验室信息化与实验技术学组委员。长期从事临床转化与前沿基础研究，聚焦细胞工程、干细胞、类器官、基因编辑与肿瘤转化研究。构建从样本处理、细胞培养、基因修饰到功能验证的全链条细胞技术平台，支撑临床疑难病理诊断、个体化治疗与转化医学研究：

血液与免疫细胞技术

主导免疫细胞高效分离与体外扩增体系优化，熟练运用流式细胞术完成高精度分选与表型鉴定；结合免疫荧光、组织化学，实现复杂微环境内细胞精准定位与定性分析，为病理诊断提供关键形态学与分子依据。

干细胞与再生医学

建立人脐带血、脐带间充质干细胞标准化分离、培养、鉴定体系，满足临床级细胞纯度与活性要求；开展人 iPS 细胞重编程、长期培养与定向诱导分化；成功构建人 / 猪子宫内膜类器官模型，为生殖医学与药物筛选提供三维体外模型；建立生殖细胞玻璃化冷冻保存技术，显著提升珍贵生物样本复苏率与存活质量。

肿瘤与基因工程技术

完成多种肿瘤细胞体外稳定培养与功能鉴定，支撑药敏筛选研究；运用CRISPR/Cas9 基因编辑，实现人 NK 细胞、猪胎儿成纤维细胞定点遗传修饰，增强免疫细胞抗肿瘤活性，为异种器官移植提供基因工程细胞株。

技术整合与临床支撑

融合细胞生物学、分子遗传学、病理学技术，有效支撑科室在疑难病理诊断、个体化治疗、前沿转化医学等关键方向的科研与临床工作。

主持省部级、国家级子课题等3项，以第一 / 通讯作者在国际主流期刊发表多篇高水平论文，参与研究发表于Nature等顶刊，涵盖卵母细胞成熟、胚胎干细胞、生殖生物学、动物育种与营养等方向。

科研项目（主持）：

重庆市技术创新与应用发展专项重点项目，CSTB2024TIAD-KPX0050，中医药特色制剂研发与应用，2025.01–2027.12，150 万元，在研

国家重点研发计划项目子课题，2023YFD1300503-3，家畜（猪、牛）早期胚胎发育阻滞机制解析，2023.12–2027.11，100 万元，在研

中央高校基本科研项目，X20240270，基于阿秒技术联合 CTCs 的肺癌早诊研发技术，2025.01–2025.12，15 万元，结题

代表性论文：

Xu Pan; Xin Li; Liang Dong; Teng Liu; Min Zhang; Lining Zhang; Xiyuan Zhang; Lingjuan Huang; Wensheng Shi; Hongyin Sun; Zhaoyu Fang; Jie Sun; Yaoxuan Huang; Hua Shao; Yeqi Wang; Mingzhu Yin; Tumour vasculature at single-cell resolution. Nature, 2024, 632(8024)

Shao H; Li R; Ma C; Chen E; X. Liu XJ; Xenopus oocyte meiosis lacks spindle assembly checkpoint control. Journal of Cell Biology, 2013, 201(2): 191-200.  

Shao H; Ma C; Zhang X; Li R; Miller AL; Bement WM; Liu XJ; Aurora B regulates spindle bipolarity in meiosis in vertebrate oocytes. cell cycle, 2012, 11(14): 2672-2680.

Huang G; Li R; Xiao J; Du T; Shao H*; A Method for Karyotyping Zebrafish Oocytes, zebrafish, 2019, 16(5): 490-492.

Hua Shao*; Jie Cao; Ronghua Lu; Enhanced differentiation capacity of parthenogenetic embryonic stem cells via incorporation of non-growing oocyte genomes in mouse. Am J Stem Cells, 2025, 14(3): 134-145.

Liu D, Shao H, Wang H, Liu XJ (2014) Meiosis I in Xenopus oocytes is not error-prone despite lacking spindle assembly checkpoint. Cell Cycle 13(10):1602-6.

Yang H, Fu D, Shao H, Kong X, Wang W, Yang X, Nyachoti CM, Yin Y. (2012) Impacts of birth weight on plasma, liver and skeletal muscle neutral amino acid profiles and intestinal amino acid transporters in suckling Huanjiang mini-piglets. PLoS One. 2012;7(12):e50921.

Tang XS，Shao H，Li TJ，Tang ZR， Huang RL，Wang SP，Kong XF，Wu X，Yin YL（2012）Dietary supplementation with bovine lactoferrampin-lactoferricin produced by Pichia pastoris fed-batch fermentation affects intestinal microflora in weaned piglets. Appl Biochem Biotechnol.168(4):887-98.

Wenkai Ren， Tiejun Li， Hua Shao，Yinghui Li， Gang Liu， Li Wu， Miaomiao Wu and Xiaosong Wu (2012) Effect of dietary L-arginine supplementation on edema disease. J Food. Agri.Envir. Vol.10(3& 4):630-634.

Fugui Yin，Jinquan Wang， Zhen Ruan，Ruilin Huang，Yongfei Wang，Hua Shao，Ju Huang,Yuyun Mu and Qingqi Wen (2012) The in vitro digestion rate and in vivo digestibility ofraw starches from selected cereals and tubers. J Food. Agri. Envi. Vol.10 (3&4):577-581

Ze-Meng Feng, Jie-Ping Guo, Xiang-Feng Kong, Yu-Long Yin, Guoqi Lian, Dezhi Fu, Hua Shao and Charler Martin Nyachoti (2012) Which housekeeping gene can be well used in gene expression analysis in Chinese local pig breeds? J. Food Agri. Envir. 10(3&4):825-833.

Jie Pan, Wence Wang, Yunchao Wang, Xiangdong Ma, Weigang Duan, Ruilin Huang, Hua Shao, Xiaosong Wu and Rejun Fan (2012). Supplementation of phytase on diet improves the apparent ileal digestibility of amino acids and P in growing piglets. J. Food Agri. Envir. 10(3&4):746-749

Dyce PW, Shen W, Huynh E, Shao H, Villagómez DA, Kidder GM, King WA, Li J. (2011) Analysis of oocyte-like cells differentiated from porcine fetal skin-derived stem cells. Stem Cells Dev.20(5):809-19

Shao H, Wei Z, Wang L, Wen L, Duan B, Mang L, Bou S (2007) Generation and characterization of mouse parthenogenetic embryonic stem cells containing genomes from non-growing and fully grown oocytes. Cell Biol Int. 31(11):1336-44.

Ren W, Li Y, Yu X, Luo W, Liu G, Shao H, Yin Y. (2013) Glutamine modifies immune responses of mice infected with porcine circovirus type 2. Br J Nutr. 110(6):1053-60.

Hua Shao, Ruizhen Li, ZhuyingWei, Fengwu Wang, Shorgan Bou. Establishment of embryonic stem (ES) cell lines derived from new-born or adult mouse ovary grafted underneath the kidney capsule. Cell Research (2006) 16:S61

Hua Shao, Ruizhen Li, Fengwu Wang, ZhuyingWei, Shorgan Bou. Isolation and characterization of embryonic stem (ES) cells directly derived from transgenic mice strongly expressing greenfluorescence protein(GFP). Cell Research (2006) 16: S61-62